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Madan, Molly
- Role of ELISA in Diagnosis of Helicobacter Pylori
Authors
1 Department of Microbiology & Medicine, Subharti Medical College, Swami Vivekanand Subhati University, Meerut, UP, IN
Source
International Journal of Contemporary Medicine, Vol 1, No 2 (2013), Pagination: 1-6Abstract
Over the last 30 years Helicobacter pylori has proved to be of overwhelming importance in the etiology of a number of common gastroduodenal diseases, like chronic gastritis, peptic ulceration and gastric cancer(1). This study was undertaken to see the association of Helicobacter pylori in cases of Peptic ulcer disease and to study the diagnostic yield of various standard conventional methods and serological test for Helicobacter pylori. Method: 150 patients with clinical suspicion of dyspepsia and 30 healthy controls were recruited over a year. Antrum biopsy pieces were taken for Rapid urease test and Gram stain. Blood sample were taken and serum was separated for detection and estimation of H. pylori IgG antibodies through ELISA. Results: Majority of the cases were presented with chronic superficial gastritis (42%) followed by duodenal ulcer (37.33%) and Non ulcer dyspepsia was seen in 67% patients of dyspepsia. H. pylori were detected in 74.0% of study group and 3.33% control by one or more methods used for diagnosis. Highest number of positive case (74%) were detected by ELISA, followed by RUT (52%) and direct microscopy (18%) whereas in control group it was 0% by direct microscopy and 3.33% (1 case) each by RUT and ELISA. Invasive cases (119) like DU and CSG were ELISA positive (79%) with a high titer (> 100RU/ml). Conclusion: IgG ELISA provides a useful non-invasive serodiagnostic test for H. pylori and may be useful as part of a screening programme to reduce endoscopy workload especially in patients with symptoms of Dyspepsia.Keywords
ELISA, Helicobacter pylori, Peptic ulcer disease, DyspepsiaReferences
- Norman S.Williams, Christopher J.K.Bulstrode, P.Ronan O’Connell. Bailey & Love’s. Short practice of surgery. 2008; 25thed (60), Stomach and duodenum: 1051-52.
- Malfertheiner P, Mc coll K, Baldi F, et al. Update on H. pylori research. Dyspepsia. Eur J Gatroention Hepatol 1997; 9:624-5.
- Talley NJ, Weaver AL, Zinsmeister AR, et al. Onset & disappearence of GI symptoms & functional GI disorders. Am J Epidemiol 1992; 136:165-77.
- Talley NJ, Zinsmeister AR, Schleck CD, et al. Dyspepsia and dyspepsia subgroups: a population based study. Gastroenterology 1992; 102: 1259-68.
- Jones RH, Lydeard SE, Hobbs FD, et al. Dyspepsia in England & scotland. Gut 1990; 3:401-5.
- Graham DY, Lew GM, Klein PD, et al. Effect of Rx of H. pylori infection on the long term recurrance of gastric / duodenal ulcer. A randomic controlled study. Ann Intern Med 1992; 116:705.
- Peterson WL, H. pylori and PUD (See comments). N. Engl J Med 1991; 324:1043-104Kitterman JA.
- EA. Role of H. pylori in duodenal ulcer Drugs 1992; 44:921-7.
- Allan D. Pronovost, Steven L. Rose, Jan W. Pawlak, Howard Robin and R. Schneider. Evaluation of a new Immunodiagnostic Assay for Helicobacter pylori detection: Correlation with histopathological and microbiological results. J of Clinical Microbiol. Jan. 1994;32(1):46-50.
- McGuigan, J. E. 1978. Peptic ulcer and gastritis, p. 1229-1248. In J. D. Wilson, et al. Harrison’s principles of internal medicine, 12th ed. McGraw- Hill, New York.
- Betty A. Forbes, Daniel F. Sahm, Alice S. Weissfeld. Bailey & Scott’s. Diagnostic Microbiology 12 ed. (36) Campylobacter, Arcobacter and Helicobacter: 421.
- T.S. Chua, K.M. Fock, E.K. Teo, T.M. Ng. Validation of 13C-Urea Breath test for the diagnosis of Helicobacter pylori infection in the Singapore population. Singapore Med J 2002; 43(8): 408-11.
- Cutler AF, Havstad S, Ma CK, Blaser MJ, Perez- Perez GI, Schubert TT. Accuracy of invasive and noninvasive tests to diagnose Helicobacter pylori infection. Gastroenterology 1995; 109:136-41.
- H. Rautelin & T. U. Kosunen. H. pylori and associated gastroduodenal diseases. APMIS 1991; 99(8): 677-95
- AC Jemilohun, JA Otegbayo, Samuel O Ola, OA Oluwasola, A Akere. Prevalence of Helicobacter pylori among Nigerian patients with dyspepsia in Ibadan. Pan African Medical J 2011; 6: 18.
- Rajesh Kumar, G Bano, B Kapoor, Sunil Sharma, Yudhvir Gupta. Clinical profile in H. pylori positive patients in Jammu. JK Science 2006; 8(3): 148-50.
- S Prasad, M Mathan, G Chandy, DP Rajan, S Venkateswaran, BS Ramakrishna, et al. Prevalence of helicobacter pylori in southern Indian controls and patients with gastroduodenal disease. J Gastroenterol Hepatol 1994; 9(5): 501-506.
- Gebbers JO, Altermatt HJ, Altorfer J. Campylobacter pylori: cause of gastritis and ulcer disease? Schweiz Med Wochenschr. 1988; 118(16): 577-583.
- H. Raskov, C. Lanng, K. Gaarslev. B Fischer Hansen, O. Hauch. Screening for Campylobacter pyloridis in patients with upper dyspepsia and the relation to inflammation of the human gastric antrum. Scand. J. of Gastro Enterol 1987; 22(5): 568-572.
- Rauws E.A, Langenberg W, Hauthoff H.J, Zanen HC, Tytgat GN. Campylobacter pyloridis – associated chronic active antral gastritis; a prospective study of its prevalence and the effects of antibacterial and anti ulcer treatment. Gastroenterology 1988; 94(1): 33-40.
- U Arora, A Aggarwal, K Singh. Comparative evaluation of conventional methods and ELISA based IgG antibodies detection for diagnosis of Helicobacter pylori infection in cases of dyspepsia. Indian J Med Microbiol 2003;21:46-8.
- Vandana Berry, Vidya Sagar. Rapid urease test to diagnose Helicobacter pylori infection. JK Science 2006;
- S. Sengupta, K. Saraswati, A Varaiya, A De, A Gogate. Helicobacter pylori in duodenal ulcer disease and its eradication. IJMM, 2002;20:3: 163-164.
- Piyalee Gupta, LB Pandey, RN Bhera, MK Rai, R Chandra. Role of ELISA in H. pylori detection and its correlation with urease test. Indian J Pathol Microbiol. 2003;46(3).
- T.C.K. Tham, N. McLaughlin, D.F. Hughes, M. Ferguson, J.J. Crosbie, M. Madden, S. Namnyak and F.A. O’Connor. Possible role of Helicobacter pylori serology in reducing endoscopy workload. Postgrad Med J; 1994; 70: 809–12.
- Profile of HIV Positive Attendees of an Integrated Counseling and Testing Centre in Meerut- a Changing Trend
Authors
1 Department of Microbiology Subharti Medical College, Swami Vivekananda Subharti University, Meerut, Uttar Pradesh
Source
Indian Journal of Public Health Research & Development, Vol 4, No 2 (2013), Pagination: 44-47Abstract
Epidemiology of HIV-AIDS in an area especially with regards to socio-demographic factors helps in choosing and implementing a particular intervention in much better way. Study included counseling and testing of clients that were either self initiated or referred from the hospital over a period of 2 years. Analysis of ICTC data of all positive attendees was done. HIV positivity rate was 1% with male to female ratio of 2.2:1. Majority of positive males were literate and belonged to service class whereas most of the positive females were housewives. Spillage of the epidemic on to general population might well cause significant alteration in trends noted till times.Keywords
HIV, Seropositive, ICTC, Attendees, CounselingReferences
- Park K. Park’s Text book of Preventive and social Medicine 19th ed. Jabalpur (India) 2007; 8:359.
- National AIDS Control Organization htpp: // www.nacoonline.org
- Kunanusont C, Phoolcharoen W, Bodaramik Y. Evolution of medical services for HIV/AIDS in Thailand. J Med Assoc Thai 1999; 82: 425–434.
- National AIDS Control Organization, Ministry of Health and Family Welfare, Govt. of India, New Delhi. Manual on Quality standards for HIV testing laboratories March 2007: 9-13
- Joardar GK, Sarkar A, Chatterjee C, Bhattacharya RN, Sarkar S, Banerjee P. Profile of attendees in the voluntary counseling and testing centre of north Bengal Medical College in Darjeeling of West Bengal. Indian Journal of Community Medicine 2006; 31:237-240
- Lal S. Current status of AIDS and HIV infection in India. J Indian Med. Association 1994; 92:3-4
- Giri TK Wali JP, Meena HS, Pande I, Uppal S, Kailash S. Sociodemographic characterstic of HIV infection in North. Indian J. Commun. Disease 1995; 27:1-9
- Mehendale SM, Shepherd ME, Divekar AD, Gangakhedkar RR, Kamble SS, Menon PA, et al. Evidence for high prevalence & rapid transmission of HIV among individual attending STD clinics Pune, India. Indian J Med Res 1996; 104:: 327-35
- Kothari K, Goyal S. Clinical profile of AIDS. J assoc Physicians India Aids Research and Review.1999; 2:7-9.
- A Study of Vancomycin Resistance in Enterococci from Urinary Specimens at a Tertiary Care Hospital in Meerut
Authors
1 Department of Microbiology, Subharti Medical College, Swami Vivekananda Subharti University, Meerut, UP, IN
Source
Indian Journal of Public Health Research & Development, Vol 4, No 3 (2013), Pagination: 39-43Abstract
Owing to the paucity of information on vancomycin resistance in enterococci from India, in the present study, enterococci isolated from urine specimen were studied for there antibiotic susceptibility & screened for vancomycin resistance.
A total of 106 enterococci isolates obtained in pure and significant numbers (>10 5 cfu/ml) from urine specimens were subjected to agar screen method of Clinical & Laboratory Standards Institute (CLSI) for presence/absence of vancomycin resistance. Minimum inhibitory concentration (MIC) was performed by agar dilution method. Antibiotic susceptibility pattern including susceptibility to high level gentamicin (120 μg/ml) was tested by Kirby Bauer disc diffusion method.
Two out of hundred & six (1.9%) isolates were vancomycin resistant enterococci (VRE). Both were Enterococcus fecalis with MIC>32 μg/ml. Although the frequency of isolation of VRE is not very high in our setting as compared to West, the problem may just be emerging. All patients with suspected UTI who have significant bacterial counts should be screened for vancomycin resistance.
Keywords
Enterococci, Vancomycin, Resistance, UrineReferences
- Schaberg DR, Zervos MJ. Intergeneric and interspecific gene exchange in gram-positive enterococci. Anti Microb Agents Chemother.1986;30:817-822.
- Murray, B.E. The life and times of the enterococcus. Clin. Microbiol.Rev.1990;3:45–65.
- Facklam RR, Collins MD. Identification of enterococcus species isolated from human infection by a conventional test scheme. J Clin Microbial 1989;27: 731-734.
- Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard- Tenth Edition CLSI document M2-A10. CLSI Wayne, Pa.
- Robert C Moellering Jr: Enterococcus species, Streptococcus bovis and Leuconostoc species. In: Principles and Practice of Infectious Disease, 5th edition (Mandell GL, Douglas RG, Bennet JE, eds).Philadelphia: Churchill Livingstone, 2000;pp2147-2166
- Low DE, Willey BM, Betschel S, Kreiswirth B: Enterococcus: pathogens of the 90’s. Eur J Surg Suppl 1994; 573:19-24.
- Faclam RR, Sahm DF and Tiexeira LM: Enterococcus In: Manual of clinical microbiology, 7th editon (Murray PR, Barron EJ, Pfaller MA, Tenover PC, Yolken RH eds). Washington DC ASM press, 1999; pp297-305.
- Huycke MM, Sahm DF, Gilmore MS. Multipledrug resistant enterococci: The nature of problem and agenda for the future. Emerg. Infect. Dis. 1998; 4:239-249
- Desai PJ, Pandit D, Mathur M, A Gogate. Prevalence, identification and distribution of various species of enterococci isolated from clinical specimens with special reference to urinary tract infection in catheterized patients. Indian Journal of Medical MICROBIOLOGY 2001;19(3):132-137.
- Randhawa V.S, Kapoor L, Singh V and Mehta G.Aminoglycoside resistance in enterococci isolated from paediatric septicaemia in a tertiary care hospital in north India. Indian J Med Res 119 (Suppl) May 2004, 77-79
- Karmarkar M.G, Gershom ES. &. Mehta P. R. Enterococcal infections with special reference to phenotypic characterization & drug resistance. Indian J Med Res 119 (Suppl) May 2004, pp 22-25.
- Bhat KG, Paul C and Bhat MG. Neonatal bacteraemia due to high level aminoglycoside resistance (HLAR) enterococci. Indian J Pediatr. 1997; Jul-Aug: 64(4):537-539
- Jesudason MV, Pratima VL, Pandian R, Abigail S. Characterization of penicillin resistant enterococci. Ind J Med Microbiol.1998.16 (1): 16-18
- Cetinkaya Y, P. Folk and C.Glen Mayhall. Vancomycin Resistant Enterococci. Clinical Microbiology reviews 2000: 13; 4; 686–707.
- Collins, L. A., Malanoski GJ, Eliopoulos GM, Wennersten CB,.Ferraro MJ, and Moellering RC. In vitro activity of RP 59500, an injectable streptogramin antibiotic, against vancomycinresistant gram-positive organisms. Antimicrob. Agents Chemother. 1993;37:598–601.
- Jones, R. N., Erwin M. E., and Anderson S. C. Emerging multiply resistant enterococci among clinical isolates: validation of E test to recognize glycopeptide resistant strains. Diagn. Microbiol. Infect. Dis. 1995; 21:95–100.
- Centers for Disease Control and Prevention. 1993. Nosocomial enterococci resistant to vancomycin—United States, 1989–1993. Morb. Mortal. Weekly Rep. 42:597–599.
- Mac Namara EB, King EM and Smyth EG. A survey of antimicrobial susceptibility of clinical isolates of enterococcus species from Irish hospitals. J Antimicrobial Chemother. 1995; 35:185-189.